Which of the Following Enzyme/substrate Combinations Is Used in the Chromogenic

question 21

Multiple Choice

Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

• A) Horseradish peroxidase with Luminol/H₂O₂/p-iodophenol
• B) Alkaline phosphatase with 1-2 dioxetane derivatives
• C) Horseradish peroxidase with disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2  -(5  -chloro) tricyclo [3.3.1.1] decan}4-yl)-1-phenyl phosphate
• D) Alkaline phosphatase with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate

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