A Hybridization Assay Is Run Under the Following Conditions: 40

question 29

Multiple Choice

A hybridization assay is run under the following conditions: 40% formamide with 0.5X SSC at 70^oC. When the bands were visualized, there were many more bands than expected in all wells on the membrane. Which of the following changes should be made to the hybridization conditions to get results that are closer to those expected?

• A) Decrease formamide
• B) Increase temperature
• C) Increase SSC concentration
• D) Decrease temperature

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Definitions:

Private Placement

A means of raising capital through the sale of securities to a small number of select investors rather than through a public offering.

Initial Public Offering

The process by which a private company becomes publicly traded by offering its shares to the public for the first time.

OSC

Typically refers to the Ontario Securities Commission, a regulatory body responsible for overseeing the capital markets in Ontario, Canada.

Exchange

Refers to a marketplace where securities, commodities, derivatives, and other financial instruments are traded.