After Chromogenic Signal Detection on a Northern Blot, Circular Colorless

question 23

Multiple Choice

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?

• A) Incomplete electrophoresis of the nucleic acid
• B) Loss of alkaline phosphatase activity
• C) Incorrect stringency conditions for probe binding
• D) Bubbles or incomplete contact between the membrane and the gel

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