In What Way Is Fluorometry More Accurate Than Spectrophotometry

question 20

Multiple Choice

In what way is fluorometry more accurate than spectrophotometry?

• A) Fluorescence is more specific for single nucleotides.
• B) Spectrophotometry only measures absorbance from intact nucleic acid polymers.
• C) Using specific stains, fluorometry only detects intact double-stranded DNA.
• D) Absorbance may require dilution of sample before reading.

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Definitions:

Dependent Variable

In an experimental setting, the variable that is being tested and measured, which is expected to change as a result of manipulations to the independent variable.

Dose-Response Relationship

The relationship between the quantity of a drug or other exposure and the magnitude of the effect it has on an organism.

Predictive Validity

The extent to which a test or measurement can accurately forecast or predict future outcomes or behaviors.

Criterion Validity

The extent to which a test's results correlate with other measures or outcomes that are already accepted as reflecting the variable being tested.