Sequence-Specific PCR Using Allele-Specific Primers and Primers for an Amplification

question 20

Multiple Choice

Sequence-specific PCR using allele-specific primers and primers for an amplification control has been performed on DNA isolated from a patient requiring bone marrow transplantation. Analysis of the reaction by gel electrophoresis reveals the presence of two bands in a gel lane. How is this interpreted?

• A) False positive
• B) False negative
• C) True positive
• D) True negative

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