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A Sanger Sequencing Procedure Has Been Performed on a 300-Bp

question 14

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A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the fragments were resolved on the gel, there were bands distributed throughout the T, C and A lanes, but in the G lane, there was one thick band at the bottom of the gel. What is the likely cause of these results?

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Definitions:

Operating Hours

The scheduled or actual hours during which a business is open to conduct operations or provide services to customers.

Double-Declining-Balance Rate

A method of accelerated depreciation that applies a constant rate, which is double the straight-line rate, to the declining book value of an asset each year.

Straight-Line Rate

A method of calculating depreciation of an asset, dividing its cost by the number of years in its useful life.

Depreciation Expense

The structured spreading of the expense associated with a tangible asset across its useful life.

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