Patient DNA Has Been Mixed in Separate Wells with a Probe

question 5

Multiple Choice

Patient DNA has been mixed in separate wells with a probe that detects the 1691G>A mutation associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is added to all wells, and the fluorescence is measured in each well. Fluorescence was detected in the well that included the normal probe, and no fluorescence was detected in the well containing the mutant probe. Which of the following assays was performed?

• A) Invader assay
• B) Nonisotopic RNase cleavage assay
• C) Chemical cleavage assay
• D) High-density oligonucleotide array

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Definitions:

Diterpene

A type of terpene consisting of four isoprene units, making up 20 carbon atoms, often found in essential oils and used in medicines and perfumes.

Prostaglandin

A collection of lipid compounds that are physiologically active and exhibit various hormone-like actions in animals.

Carboxyl Group

A functional group consisting of a carbon atom double-bonded to an oxygen atom and single-bonded to a hydroxyl group, denoted as -COOH in organic compounds.

Prostaglandins

A group of physiologically active lipid compounds having diverse hormone-like effects in animals, derived enzymatically from fatty acids.