Nucleic Acid Has Been Isolated from a Urethral Swab and a PCR

question 6

Multiple Choice

Nucleic acid has been isolated from a urethral swab and a PCR is performed on the sample to detect Neisseria gonorrhoeae. The positive control has a band at the expected size; the negative template control and the contamination controls do not have bands; and the amplification control has a band of expected size. The patient sample has a band consistent with the amplification control band and does not have a band corresponding to the positive control band. How is this result interpreted?

• A) The patient has a positive PCR result for Neisseria gonorrhoeae.
• B) The results are not acceptable because the control results are invalid.
• C) The patient does not have Neisseria gonorrhoeae, and it is a true negative result.
• D) The patient has a false negative result for Neisseria gonorrhoeae.

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