A Molecular Technologist Has Run a Vertical Gel

question 4

Multiple Choice

A molecular technologist has run a vertical gel. While analyzing the gel, the technologist notices that the bands in the outer lanes did not run as far as similar-sized bands in the inner lanes, so the bands look like a smile on the gel. What is the explanation for this?

• A) Samples were loaded into the inner wells first and got a head start moving down the gel.
• B) A larger volume of sample was loaded into the outer wells so that they ran slower.
• C) The electrodes in the middle of the gel were working better than at the edges of the gel.
• D) The temperature across the gel was not constant, so the inner wells were warmer.

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