Below Is a 2D Chromatogram That Shows the Separation of Five

question 14

Multiple Choice

Below is a 2D chromatogram that shows the separation of five amino acids. In this technique, the amino acid mixture is first separated by chromatography using a polar solvent system. Then the plate is rotated 90°, and the amino acids are further separated by electrophoresis. Identify the spots obtained from a mixture of Trp, Glu, Lys, Ile and Thr. A table with isoelectric points is included to aid in solving this problem. amino acid pI value
Trp
5) 9
Glu
3) 2
Lys
9) 7
Ile
6) 0
Thr
5) 6

• A) 1=Lys, 2=Thr, 3=Glu, 4=Trp, 5=Ile
• B) 1=Glu, 2=Thr, 3=Lys, 4=Trp, 5=Ile
• C) 1=Lys, 2=Ile, 3=Glu, 4=Trp, 5=Thr
• D) 1=Glu, 2=Ile, 3=Lys, 4=Trp, 5=Thr

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Definitions:

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