A Protein Contains Two Cysteine Residues That Are Widely Separated

question 69

Multiple Choice

A protein contains two cysteine residues that are widely separated in its amino acid sequence but form a disulfide bridge in its tertiary structure.Which is the most likely outcome if one of these cysteine residues is replaced with an alanine residue?

• A) The tertiary structure will remain unchanged under normal physiological conditions and will also retain its stability under denaturing conditions.
• B) The tertiary structure will remain unchanged under normal physiological conditions but will lose it ability to resist unfolding under denaturing conditions.
• C) The tertiary structure will be disrupted slightly under normal physiological conditions but will retain its stability under denaturing conditions.
• D) The tertiary structure will be greatly disrupted under normal physiological conditions but will retain its stability under denaturing conditions.
• E) The tertiary structure will be greatly disrupted under normal physiological conditions and will lose it ability to resist unfolding under denaturing conditions.

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