A Researcher Studying a Line of Mouse Skin Cells Has

question 214

Multiple Choice

A researcher studying a line of mouse skin cells has identified an oligosaccharide group attached to the cells' surfaces.What experiment should the researcher perform to test whether this oligosaccharide is important in cell-cell recognition?

• A) Isolate membrane proteins from the mouse skin cells, and determine what percentage of these contain the oligosaccharide being investigated.
• B) Introduce genetic mutations that prevent synthesis of all membrane proteins, and observe whether this results in loss of the mouse skin cells' ability to recognize other cells.
• C) Attach a fluorescent probe to a protein known to bind to the oligosaccharide, then incubate the protein with mouse skin cells and observe the cells using fluorescence microscopy.
• D) Run a broad survey of many other types of cells to see which cells express this oligosaccharide and are able to bind to the mouse skin cells being investigated.
• E) Treat the mouse skin cells with enzymes that hydrolyze bonds between sugar molecules, and observe whether treated cells no longer recognize other cells.

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