Refer to the Figure and Table Below

question 144

Multiple Choice

Refer to the figure and table below. A researcher treated mouse kidney cells growing in culture so that they took up a fluorescent dye into their cytosols without losing viability.(This dye undergoes a change in fluorescence intensity upon binding calcium ions.) The treated cells were then incubated with and without a compound thought to act as a signaling molecule, and the cells were observed for changes in fluorescence.The researcher hypothesizes that the signaling molecule functions according to the pathway shown in the diagram above.As a next step, the researcher wants to acquire evidence that IP₃ is involved in this pathway.Which procedure will provide needed evidence about IP₃ as the entire experiment is repeated?

• A) Using mouse kidney cells containing a mutation that knocks out GTP binding by G protein
• B) Using mouse kidney cells containing a mutation that knocks out phospholipase C
• C) Adding a calcium channel inhibitor during all trials
• D) Replacing the signaling molecule with an agonist molecule
• E) Replacing the signaling molecule with IP₃

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