Which Experimental Technique Would Be Best Used to Investigate Whether

question 125

Multiple Choice

Which experimental technique would be best used to investigate whether a transcription factor is activated as part of a signal transduction pathway?

• A) Measure changes in the activities of cytosolic enzymes in response to stimulation of the pathway.
• B) Isolate mutants that do not bind signal molecule, and characterize the mutations in the DNA.
• C) Compare the cell's messenger RNA molecules before and after stimulation of the pathway.
• D) Measure intracellular calcium ion concentration changes in response to stimulation of the pathway.
• E) Label the signal molecule using the radioisotope carbon-14, and observe whether the carbon-14 radiolabel enters the cell.

---

Definitions:

Therapeutic Interventions

Actions or strategies used by health professionals to assist individuals in overcoming physical or mental health issues.

Cooperation

The act or process of working together towards common goals or benefits, often resulting in shared success or outcomes.

Trait

A stable characteristic or quality that describes a person's typical behavior or attitude.

Spontaneous Remission

The unexpected improvement or cure of a disease or condition without medical intervention, often seen in cases such as cancer.