Which Experimental Technique Would Be Best Used to Investigate Whether

question 125

Multiple Choice

Which experimental technique would be best used to investigate whether a transcription factor is activated as part of a signal transduction pathway?

• A) Measure changes in the activities of cytosolic enzymes in response to stimulation of the pathway.
• B) Isolate mutants that do not bind signal molecule, and characterize the mutations in the DNA.
• C) Compare the cell's messenger RNA molecules before and after stimulation of the pathway.
• D) Measure intracellular calcium ion concentration changes in response to stimulation of the pathway.
• E) Label the signal molecule using the radioisotope carbon-14, and observe whether the carbon-14 radiolabel enters the cell.

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Definitions:

Injunctive Norms

Social norms that are perceived as approved or disapproved behaviors by a cultural or social group, guiding individuals on what they ought to do.

Focus Theory

An approach in psychology that examines how individuals concentrate their attention or energy on specific thoughts, tasks, or goals and the impact of this focus on behavior and cognition.

Social Contagion

The spread of ideas, attitudes, or behaviors through a group or society via social influence, similar to the transmission of infectious diseases.

Informational Influence

The effect on an individual's behavior or opinions due to the desire to be correct, often resulting from the acceptance of information from others as evidence about reality.