A Biochemist Generates an Enzyme Activity Curve for an Unknown

question 164

Multiple Choice

A biochemist generates an enzyme activity curve for an unknown enzyme.The curve is shown below. Which statement best explains the shape of this curve?

• A) The enzyme has multiple active sites. As the enzyme binds substrate at one site, its affinity for binding substrate at other active sites increases.
• B) The enzyme has a single active site. As the enzyme binds substrate, the activity of the enzyme stalls until it can clear the active site.
• C) The enzyme has multiple active sites. As the substrate concentration increases, all sites must be equally bound for the reaction to proceed.
• D) The enzyme has multiple active sites. Substrate binding at each of the sites is dependent upon the temperature of the reaction.
• E) The enzyme has a single active site. Substrate binding at this particular site occurs within a narrow pH range, termed the equivalence point.

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