Refer to the Figure

question 165

Multiple Choice

Refer to the figure. Through the use of linkage analysis, a researcher has identified a candidate gene responsible for a novel neurological disease.To obtain enough sample for DNA fingerprinting, the researcher uses PCR to amplify the gene.From the parent's DNA, PCR amplifies one band.From the child's DNA, the same size band plus a second larger band is amplified.Based on the published sequence of the gene, the researcher knows that restriction enzyme A will cut the wild-type allele into two fragments.The fragment sizes identified by DNA gel electrophoresis are shown in the figure.What technique should the researcher next use to identify the nature of the mutation with certainty?

• A) DNA fingerprinting
• B) DNA linkage analysis
• C) DNA sequencing
• D) Gel electrophoresis
• E) Genetic screening

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