Suppose One Restriction Enzyme (Enzyme A, Which Targets a Given

question 144

Multiple Choice

Suppose one restriction enzyme (enzyme A, which targets a given sequence) makes one cut in a linear DNA fragment, and another restriction enzyme (enzyme B, which targets another distinct sequence) makes another cut in that same DNA.The cuts are not in the same place, and no two cut fragments are exactly the same size.The restriction digest is set up on an electrophoresis gel.How many bands should appear on the gel if both enzymes, A and B, are used in the restriction digest?

• A) one
• B) two
• C) four
• D) three
• E) five

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Definitions:

Fossil Fuels

Natural fuels formed from the remains of living organisms, such as coal, oil, and natural gas.

Gas Hydrate

An icelike solid mixture of water and a natural gas, usually methane.

Coal-bed Methane

A type of natural gas extracted from coal beds, often used for heating and generating electricity.

Oil Shale

A kerogen-bearing, thinly layered, fine-grained sedimentary rock that yields liquid or gaseous hydrocarbons upon heating and distillation.