Suppose You Run Gel Electrophoresis Using the Standard Voltage, but the Spaces

question 42

Multiple Choice

Suppose you run gel electrophoresis using the standard voltage, but the spaces between the polymers of the gel are smaller than usual because of an error in making the gel.The samples are commonly run, so you neglect to include a lane with standards that would indicate the sizes of the bands.What is the most likely result?

• A) The DNA bands will migrate more quickly across the gel and will appear to be larger than they are.
• B) The DNA bands will migrate more quickly across the gel and will appear to be smaller than they are.
• C) The DNA bands will migrate more slowly across the gel and will appear to be larger than they are.
• D) The DNA bands will migrate more slowly across the gel and will appear to be smaller than they are.
• E) The spaces between the polymers will not affect how quickly DNA migrates across the gel.

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