After Running Gel Electrophoresis on Restriction Enzyme-Digested DNA, You Have

question 98

Multiple Choice

After running gel electrophoresis on restriction enzyme-digested DNA, you have the expected results but observe that the band you are interested in is rather light.How would you make it darker using the same sample source?

• A) Run the gel again for a longer period of time
• B) Run the gel again for a shorter period of time
• C) Digest the DNA again, then run the gel again
• D) Run the gel again with less DNA
• E) Run the gel again with more DNA

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Definitions:

Semen

A bodily fluid containing spermatozoa, which is expelled during ejaculation and carries sperm for potential fertilization.

Ejaculated

Ejaculated refers to the expulsion of semen from the penis during orgasm in males.

Fluid Ounces

A unit of volume used primarily in the United States to measure liquids, equal to approximately 29.57 milliliters.

Gonadotropin-Releasing Hormone (GnRH)

A hormone that stimulates the pituitary gland to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), initiating the follicular phase of the ovarian cycle.