Suppose a Long Sequence of DNA Is Cut with Four

question 64

Multiple Choice

Suppose a long sequence of DNA is cut with four different restriction enzymes.Which restriction enzyme should produce the fewest fragments?

• A) One that cuts at the sequence GACT
• B) One that cuts at the sequence GCCTCT
• C) One that cuts at the sequence AGTTCTAT
• D) One that cuts at the sequence CAGTTCTATG
• E) All of these enzymes would produce roughly the same number of fragments.

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Definitions:

5' Cap

A modified form of guanine nucleotide added to the 5' end of eukaryotic mRNA, playing a role in mRNA stabilization and initiation of translation.

Poly-A Tail

A sequence of adenine nucleotides added to the 3' end of newly synthesized messenger RNA molecules.

Prokaryotes

Simple, unicellular organisms without a nucleus, including bacteria and archaea, characterized by the absence of membrane-bound organelles.

Eukaryotes

Organisms whose cells contain a nucleus enclosed within membranes, distinguishing them from prokaryotes.