A DNA Sequence Is Cut by Two Different Restriction Enzymes

question 170

Multiple Choice

A DNA sequence is cut by two different restriction enzymes (1 and 2) .
Digestion from enzyme 1 yields the following fragments (read in the 5ʹ-to-3ʹ direction) : CGATAC, GTCGTCGCC, and GTTATCGCGAC.Digestion from enzyme 2 yields the following fragments (read in the 5ʹ-to-3ʹ direction) : CGACGTCGTCGCC and CGATACGTTATCG.
Which sequence does enzyme 1 recognize as a site at which to cut?
(Note that the enzyme cuts in the middle of its recognition sequence.)

• A) GTTA
• B) ACGT
• C) TGGA
• D) CGCG
• E) GTCG