Refer to the figure showing high-throughput sequencing. 
At which step does PCR take place?
Definitions:
New Problems
Issues or challenges that have not been previously encountered or recognized.
Confirmation Bias
The tendency to interpret new evidence as confirmation of one's existing beliefs or theories.
Proposition
A statement or assertion that expresses a judgment or opinion, or in logic, a statement that can be either true or false.
Unit of Meaning
The smallest segment of a sentence or discourse that conveys a specific idea or meaning.
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