Below Are Four Overlapping Reads from Sequencing

question 219

Multiple Choice

Below are four overlapping reads from sequencing.(Note: All reads are in a 5′-to-3′ direction.) Read 1: AGCGCTAAAGACGGCTCGAGTCAGCTAGTAGAA
Read 2: TGCGTAGCTATCGCATACGCAAGCGCTAAA
Read 3: GCTAGTAGAATCGATATCGAGGATCGATTAG
Read 4: GGATCGATTAGAGCTATAGTAGGCTGATGAC
Which is the most likely order of these reads?

• A) 1, 2, 3, 4
• B) 1, 2, 4, 3
• C) 2, 1, 3, 4
• D) 2, 1, 4, 3
• E) 3, 4, 2, 1