Five prokaryote species (labeled A-E) have been sequenced.Refer to the table with the findings of the genes they contain.(Note: "x" denotes the species possessing a functional copy of the gene.) Gene 1 is essential for the synthesis of several amino acids.Gene 2 encodes a protein involved in strengthening the cell wall.Gene 3 produces an enzyme that breaks down methane.The functions of the other four genes are currently unknown. 
Based on these data, gene _______ is most likely to be included in the minimal genome.
Definitions:
Excessive Rotor Runout
A condition where a brake rotor deviates from its axis of rotation, leading to uneven brake wear and potential vibration during braking.
Brake Pedal Pulsation
A condition in which the brake pedal moves up and down or vibrates under the driver's foot during braking, often indicative of rotor issues.
Caliper Bore
The cylindrical opening in a brake caliper where the piston moves, playing a critical role in the braking system of a vehicle.
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