The Sequence 5ʹ-TCATGT-3ʹ Is Not a Restriction Enzyme Palindromic Recognition

question 185

Multiple Choice

The sequence 5ʹ-TCATGT-3ʹ is not a restriction enzyme palindromic recognition sequence.What mutational change would most likely make it into one?

• A) Changing T to C at the 5ʹ end
• B) Changing T to A at the 5ʹ end
• C) Changing T to G at the 5ʹ end
• D) Changing T to G at the 3ʹ end
• E) Changing T to C at the 3ʹ end

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Definitions:

Evolutionary Advantage

A genetic trait that improves an organism's ability to survive and reproduce in its environment, thereby increasing its chances of passing on its genes to the next generation.

Fructose-1,6-bisphosphate

A key sugar substrate in the glycolytic pathway, which provides energy to cells by breaking down glucose.

Glycolysis

The metabolic pathway that breaks down glucose to pyruvate, generating energy in the form of ATP and NADH, and is the first step in cellular respiration.

Citric Acid Cycle

A series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and water.