A Restriction Enzyme Recognizes the Sequence 5ʹ-GTCATGAC-3ʹ and Makes Staggered

question 154

Multiple Choice

A restriction enzyme recognizes the sequence 5ʹ-GTCATGAC-3ʹ and makes staggered cuts.Which statement is most likely to be true?

• A) Sticky ends should arise because the enzyme makes staggered cuts.
• B) Sticky ends should arise because the restriction site starts with a G.
• C) Sticky ends should arise because the restriction site is more than six nucleotides long.
• D) Blunt ends should arise because the enzyme makes staggered cuts.
• E) Blunt ends should arise because the restriction site is more than six nucleotides long.