Suppose One Restriction Enzyme (Enzyme A) Makes Two Cuts in a Circular

question 122

Multiple Choice

Suppose one restriction enzyme (enzyme A) makes two cuts in a circular DNA molecule, and another restriction enzyme (enzyme B) makes one cut in that same DNA.The cuts are not in the same place, and the cut fragments are not exactly the same size.The restriction digestion is set up on an electrophoresis gel.How many bands should the gel pick up if both enzymes A and B are used in the restriction digestion?

• A) Two
• B) Three
• C) Four
• D) Five
• E) Six

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Definitions:

Intestinal Infection

An infection in the intestines caused by bacteria, viruses, or parasites leading to symptoms like diarrhea and abdominal pain.

Stool Specimen

A sample of feces collected for medical testing to diagnose disorders of the digestive tract, infections, or other conditions.

Abdominal Flat Plate

A diagnostic X-ray image of the abdominal area without the use of contrast media.

Fluid

A substance that can flow and change shape such as water, essential for bodily functions like hydration and nutrient transport.