A Researcher Inserts a DNA Segment at the BamHI Recognition

question 160

Multiple Choice

A researcher inserts a DNA segment at the BamHI recognition site within a plasmid; this site is located within the tetracycline resistance gene.This plasmid also has a gene for ampicillin resistance.Following DNA transformation, the researcher must select the bacteria that have taken up the recombinant DNA and not select those that either have taken up the DNA segment only or have taken up nonrecombinant plasmids.In order to do so, the researcher should select the bacteria that

• A) are sensitive to both antibiotics.
• B) will grow on ampicillin but are sensitive to tetracycline.
• C) are resistant to both antibiotics.
• D) will grow on tetracycline but are sensitive to ampicillin.
• E) grow only on an enriched medium.

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