Suppose One Restriction Enzyme (Enzyme A) Makes Three Cuts in a Circular

question 230

Short Answer

Suppose one restriction enzyme (enzyme A) makes three cuts in a circular DNA molecule, and another restriction enzyme (enzyme B) makes one cut in that same DNA.The cuts are not in the same place, and the cut fragments are not exactly the same size.The restriction digestion is set up on an electrophoresis gel.If only enzyme A is used in the restriction digestion, the number of bands the gel should pick up is _______.

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