A Researcher Inserts a DNA Segment at the BamHI Recognition

question 137

Multiple Choice

A researcher inserts a DNA segment at the BamHI recognition site within a plasmid; this site is located within the ampicillin resistance gene.This plasmid also has a gene for tetracycline resistance.Following DNA transformation, the researcher must differentiate the bacteria that have taken up the recombinant DNA from those that have taken up either the DNA segment only or intact plasmids.In doing so, the researcher should select the bacteria that

• A) will grow on tetracycline but are sensitive to ampicillin.
• B) are sensitive to both antibiotics.
• C) are resistant to both antibiotics.
• D) will grow on ampicillin but are sensitive to tetracycline.
• E) will grow only on an enriched medium.

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Definitions:

Operating Activities

The day-to-day activities of a business involved in producing and delivering its products and services, generating cash flows from these primary activities.

Income Tax Expense

The total amount of income tax a company is obligated to pay to the government, based on its taxable income for a particular period.

Cash Basis

An accounting method where revenues and expenses are recognized only when cash is received or paid out, respectively.

Internal Rate

This term might be incomplete or unclear; however, it is possibly referring to the internal rate of return (IRR), which is a metric used in financial analysis to estimate the profitability of potential investments.