You Are Performing a Recombinant DNA Experiment Using a Restriction

question 87

Multiple Choice

You are performing a recombinant DNA experiment using a restriction enzyme that makes staggered cuts and using the GFP gene as a reporter.The plasmid DNA gets cut properly, but the different pieces do not rejoin properly.Most likely, you have a defective

• A) CAS9 protein.
• B) restriction enzyme.
• C) DNA ligase.
• D) GFP.
• E) replicon.

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