A Researcher Inserts a DNA Segment at the BamHI Recognition

question 137

Multiple Choice

A researcher inserts a DNA segment at the BamHI recognition site within a plasmid; this site is located within the ampicillin resistance gene.This plasmid also has a gene for tetracycline resistance.Following DNA transformation, the researcher must differentiate the bacteria that have taken up the recombinant DNA from those that have taken up either the DNA segment only or intact plasmids.In doing so, the researcher should select the bacteria that

• A) will grow on tetracycline but are sensitive to ampicillin.
• B) are sensitive to both antibiotics.
• C) are resistant to both antibiotics.
• D) will grow on ampicillin but are sensitive to tetracycline.
• E) will grow only on an enriched medium.

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