A Researcher Is Interested in Determining What Nucleotide Substitutions in TTX-Resistant

question 12

Multiple Choice

A researcher is interested in determining what nucleotide substitutions in TTX-resistant puffer fish sodium channels are critical for resistance.To facilitate her research, she uses PCR-based in vitro evolution to generate altered versions of the puffer fish sodium channels.To increase the possibility of generating genes that are not resistant to TTX, the researcher should

• A) use a DNA polymerase that has low fidelity.
• B) not denature the DNA in between PCR cycles.
• C) use DNA from other species of fish.
• D) use DNA only from puffer fish that do not produce TTX.
• E) limit the rounds of PCR to less than six.

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