In a Single Polymerase Chain Reaction (PCR) Cycle Consisting of 15

question 6

Multiple Choice

In a single polymerase chain reaction (PCR) cycle consisting of 15 seconds at 94°C, 30 seconds at 50°C, and 1 minute at 72°C, what is happening in the step run at 50°C?

• A) The DNA to be amplified is being denatured.
• B) Primers are being denatured.
• C) DNA polymerase is extending new DNA from the primers.
• D) Primers are annealing to the DNA to be amplified.
• E) DNA polymerase is being inactivated.

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