Twenty-Five Years Ago, When Oshima and Colleagues Discovered That a Mutation

question 30

Multiple Choice

Twenty-five years ago, when Oshima and colleagues discovered that a mutation in the GAL4 gene led to the inability to synthesize all five enzymes required for galactose catabolism (breakdown) , they couldn't be blamed for wanting to apply a bacterial model to explain this finding. What they expected, but did not find, was ________.

• A) that all five genes constitute an operon
• B) five widely separated genes, each containing a GAL4-binding site in its regulatory region
• C) that chromatin decondensation played an important role in regulating the genes of galactose catabolism
• D) that transcription is important in regulating the genes of galactose catabolism

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