In Thermal Denaturation of Double-Stranded DNA

question 8

Multiple Choice

In thermal denaturation of double-stranded DNA

• A) the phosphodiester bonds undergo hydrolysis.
• B) the absorption at 260 nm is decreased when the hydrogen-bonded bases become separated.
• C) G-C pairs are the main contributors towards the hyperchromic effect observed upon thermal denaturation.
• D) the value of Tm is usually dependent on the G + C content of the DNA.
• E) the reaction is time and concentration dependent.

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