DNA Sequencing by the Sanger Technique Makes Use of Dideoxynucleotides

question 100

Multiple Choice

DNA sequencing by the Sanger technique makes use of dideoxynucleotides for which of the following reasons?

• A) To ensure that DNA is synthesized from only one of the two DNA strands
• B) To terminate DNA synthesis at every nucleotide
• C) To promote base-pairing between the newly synthesized strand and the template strand
• D) To provide sites at which DNA cleavage will occur

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