Which of the Following Techniques for Purifying Proteins Relies on Differences

question 107

Multiple Choice

Which of the following techniques for purifying proteins relies on differences in the sizes of proteins?

• A) Ion exchange chromatography
• B) Isoelectric precipitation
• C) Salting out
• D) Gel permeation chromatography
• E) Zonal electrophoresis

---

Definitions:

Diastereomers

Stereoisomers that are not mirror images of each other, differing in the configuration at one or more (but not all) of the chiral centers in molecules.

Chiral

Describes a molecule that is not superimposable on its mirror image, often leading to different reactions or interactions for each form.

Diastereomer

Molecules that are stereoisomers, not mirror images of each other and have more than one chiral center.

Diastereomers

Isomers of the same stereo configuration that differ in their physical properties and do not act as mirror images of each other.