Screening a Recombinant DNA Library Using an 125I-Labeled Antibody Prepared

question 34

Multiple Choice

Screening a recombinant DNA library using an 125I-labeled antibody prepared against a eukaryotic protein

• A) requires that cDNA inserts have been used in the library being screened.
• B) requires the utilization of vector sequences that provide high efficiency translation of the cloned insert DNA including signals for initiation and termination of translation.
• C) requires the utilization of vector sequences that provide high efficiency transcription of the cloned insert DNA including signals for initiation and termination of transcription.
• D) will isolate any gene that encodes a protein product recognized by the antibody.
• E) all of the above statements are true.

---

Definitions:

Retirement

The act of leaving one's job and ceasing to work, typically due to age or health reasons, often requiring financial planning.