Construction of a Plasmid Containing a Human Beta Globin CDNA

question 26

Multiple Choice

Construction of a plasmid containing a human beta globin cDNA may involve all of the following EXCEPT

• A) isolation of human beta globin mRNA.
• B) use of poly dT as a primer for reverse transcriptase.
• C) transcription with
• D) homopolymer tailing of the cDNA and ligation of the tailed cDNA to the vector.
• E) coli RNA polymerase.

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