Screening a Recombinant DNA Library Using an 125I-Labeled Antibody Prepared

question 34

Multiple Choice

Screening a recombinant DNA library using an 125I-labeled antibody prepared against a eukaryotic protein

• A) requires that cDNA inserts have been used in the library being screened.
• B) requires the utilization of vector sequences that provide high efficiency translation of the cloned insert DNA including signals for initiation and termination of translation.
• C) requires the utilization of vector sequences that provide high efficiency transcription of the cloned insert DNA including signals for initiation and termination of transcription.
• D) will isolate any gene that encodes a protein product recognized by the antibody.
• E) all of the above statements are true.

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Definitions:

Shareholders

Individuals or entities that own shares in a corporation, thus having a claim on part of its assets and earnings.

Legally Liable

Being subject to legal responsibility or obligation, particularly in the context of lawsuits or compensations.

Sole Proprietorship

A business ownership structure where a single individual owns, operates, and is responsible for all aspects of the business.

Personally Liable

The legal responsibility of an individual to compensate for debt or obligation from personal assets, not through corporate or shared assets.