In the Preparation of Labeled Probes, Which Steps Do Nick-Translation

question 38

Multiple Choice

In the preparation of labeled probes, which steps do nick-translation and random oligonucleotide priming have in common?

• A) Labeling reactions are initiated by synthetic oligonucleotides.
• B) The incorporation of labeled precursor involves an in vitro DNA replication reaction.
• C) Nuclease activity can degrade newly synthesized, labeled DNA.
• D) They both incorporate -labeled ribonucleotides.

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