All of the Following Statements About the Michaelis Constant Are

question 82

Multiple Choice

All of the following statements about the Michaelis constant are correct EXCEPT which one?

• A) For many enzymes, Km approximates the equilibrium constant for the conversion of ES to E and P.
• B) A low value for Km is generally associated with a high affinity of an E for an S.
• C) Km is calculated from the intersection, in a Lineweaver-Burk plot, of the experimentally determined line with the horizontal axis.
• D) Km may be defined as the substrate concentration which gives half-maximal velocity.
• E) At a substrate concentration equal to Km, half of the total number of enzyme molecules may be present as an ES complex.

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