The List Below Contains Steps for Cloning a Gene of Interest

question 70

Multiple Choice

The list below contains steps for cloning a gene of interest. They are in random order, and some may be incorrect. Which sequence places the correct stems in the correct order?
1) Transform the DNA into E. coli.
2) Ligate cut genomic DNA fragments and cut plasmid DNA together using DNA ligase.
3) Digest the plasmid cloning vector with the same restriction enzyme.
4) Isolate genomic DNA and digest that DNA with a restriction enzyme.
5) Spread bacterial cells on medium containing lactose and ampicillin and incubate to allow colonies to grow.
6) Spread the bacterial cells on growth medium containing ampicillin and X-gal and incubate to allow colonies to grow.

• A)4, 3, 2, 1, 5
• B)4, 2, 3, 1, 5
• C)4, 2, 3, 1, 6
• D)4, 3, 2, 1, 6

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