The List Below Contains Steps for Cloning a Gene of Interest

question 70

Multiple Choice

The list below contains steps for cloning a gene of interest. They are in random order, and some may be incorrect. Which sequence places the correct stems in the correct order?
1) Transform the DNA into E. coli.
2) Ligate cut genomic DNA fragments and cut plasmid DNA together using DNA ligase.
3) Digest the plasmid cloning vector with the same restriction enzyme.
4) Isolate genomic DNA and digest that DNA with a restriction enzyme.
5) Spread bacterial cells on medium containing lactose and ampicillin and incubate to allow colonies to grow.
6) Spread the bacterial cells on growth medium containing ampicillin and X-gal and incubate to allow colonies to grow.

• A)4, 3, 2, 1, 5
• B)4, 2, 3, 1, 5
• C)4, 2, 3, 1, 6
• D)4, 3, 2, 1, 6

---

Definitions:

Evaluate Risk

The process of identifying, analyzing, and determining the level of risk associated with a specific action or scenario.

Duty to Investigate

An obligation, often legal, to conduct a formal inquiry or examination into a matter or claim.

Insurer

A company or entity that provides insurance coverage to individuals or organizations against financial loss in exchange for premium payments.

Claim

A formal assertion of one's right to money, property, or legal remedy due to harm or wrongdoing experienced.