You Are a Lab Technician Trying to Construct a Line

question 94

Multiple Choice

You are a lab technician trying to construct a line of cells that secrete a monoclonal antibody. You immunize mice with antigen, isolate B cells from the spleen, and fuse them with myeloma cells. After several weeks of screening and growing cells, you are no closer to a monoclonal antibody cell line, and that several of the cultures that are growing rapidly are not hybridomas. You also find several cultures that are slowly dying. You suspect a problem with your fusion step, so you order new myeloma cells. It is expensive to immunize mice, so you have to pick a few of the cultures to fuse with your new myeloma cells. Which of the following cells would you pick?

• A) The rapidly-growing cells
• B) The slowly-dying cells
• C) A mix of the rapidly-growing and slowly-dying cells
• D) Either culture would work, since the problem is with the fusion, not the cells themselves.
• E) None of the above; you must start the experiment over.

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Definitions:

Statistical Techniques

Mathematical methods applied to data for the purpose of analysis, interpretation, and presentation, used in various fields for informed decision-making.

Capable Process

A manufacturing or production process with the ability to produce outputs within pre-defined specifications and quality criteria reliably.

Operating Characteristics Curve

A graphical representation showing the behavior of a system, such as its true-positive rate versus false-positive rate, under various conditions.

Population Percent Defective

The percentage of items in a given population that do not meet the specified quality criteria, used as a measure of process quality.