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Use the following to answer questions:
In a hypothetical experiment, researchers split a bacterial colony in two, allowing each of the new colonies to evolve separately. They then sequenced a gene from colony 1 and colony 2.
-In the common ancestor, the nucleotide at site 327 is G. In colony 1, it changes to C; in colony 2, it also changes to C. This is an example of a _______ substitution.


Definitions:

Psychological Discomfort

A state of emotional or mental unease, often the result of cognitive dissonance, anxiety, or other psychological factors.

Choices

The act of selecting among multiple options or paths based on preferences or circumstances.

Emotions

Complex psychological states that arise from one's circumstances, mood, or relationships with others, affecting human behavior and decision-making.

Moods

Emotional states that are less intense and longer-lasting than emotions, and often without a specific identifiable cause.

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