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Use the following to answer questions:
In a hypothetical experiment, researchers split a bacterial colony in two, allowing each of the new colonies to evolve separately. They then sequenced a gene from colony 1 and colony 2.
-In the common ancestor, the nucleotide at site 167 is T. In colony 1, it changes to C; in colony 2, it remains T. This is an example of a _______ substitution.


Definitions:

Unconditional Surrender

A military surrender in which no guarantees are given to the surrendering party.

Assembly-Line Process

A manufacturing method in which a product is assembled step by step as it moves along a conveyor belt or through a series of workstations.

Mass Production

A method of producing goods in large quantities at low cost per unit, typically using assembly line techniques and standardized parts.

Second Front

In a military context, opening a new operational area of combat to distract or relieve pressure on an existing battlefront.

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