A Method Used to Insert or Transform Cells with a Plasmid

question 32

Multiple Choice

A method used to insert or transform cells with a plasmid is to:

• A) add the DNA to bacterial cells that have been lightly treated with lysozyme to produce "holes" in the cell wall.
• B) add the DNA to a heated suspension of cells at 42°C.
• C) treat the bacteria with Ca²⁺, add the DNA, and briefly heat to 42°C.
• D) incubate the DNA with the cells overnight at 4°C.
• E) mixing plasmids with an extract of broken cells.

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Definitions:

Social Pressure

The influence exerted by a society or social group on individuals to conform to expected norms and behaviors.

Public Behavior

relates to how individuals act and interact in social settings and public places, governed by societal norms and etiquette.

Compliance

The act of conforming to a request or demand, often from a figure of authority.

Compliance Tactics

Strategies used to persuade or influence others to agree with or obey a request or command.